Human HSP90aA1(Heat Shock Protein 90kDa Alpha A1) ELISA Kit

Human HSP90aA1(Heat Shock Protein 90kDa Alpha A1) ELISA Kit

Size

96T

Product ID

ELK7471

Cost

526 EUR

Alternative Names

HSP86; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp89; Hsp90; LAP2; Lipopolysaccharide-associated protein 2; Renal carcinoma antigen NY-REN-38

Assay Type

Sandwich

Sensitivity

0.65ng/mL

Standard

100ng/mL

Detection range

1.56-100ng/mL

Assay length

3h

Research Area

Signal transduction;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Alpha A1 (HSP90αA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Alpha A1 (HSP90αA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Alpha A1 (HSP90αA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Alpha A1 (HSP90αA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Description

The HSP90aA1(Heat Shock Protein 90kDa Alpha A1) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Heat-shock Proteins!