Rat HSPb2(Heat Shock Protein Beta 2) ELISA Kit

Rat HSPb2(Heat Shock Protein Beta 2) ELISA Kit

Size

96T

Product ID

ELK1495

Cost

608 EUR

Alternative Names

HSP-B2; MKBP; HSP27; Heat Shock 27kDa Protein 2; DMPK-binding protein

Assay Type

Sandwich

Sensitivity

0.055ng/mL

Standard

10ng/mL

Detection range

0.156-10ng/mL

Assay length

3h

Research Area

Signal transduction;Tumor immunity;Developmental science;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 2 (HSPβ2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 2 (HSPβ2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 2 (HSPβ2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 2 (HSPβ2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus

Heat-shock Proteins!