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HSC70/HSP70, heat shock cognate protein 70/heat shock protein 70

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Lyophilized

Immunogen

HSC70 purified from wheat germ by ATP affinity chromatography

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 100 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB), ELISA (ELISA), immunoprecipitation (IP)

Connected products

AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic, rabbit antibodies AS08 348 | anti-HSP70 | heat shock protein 70, chloroplastic , rabbit antibodies AS08 347 | anti-HSP70 | heat shock protein 70, mitochondrial, rabbit antibodies AS05 086 | anti-UGPase (cytoplasm marker), rabbit antibodiesCollection of antibodies to heat shock proteins

Recommended dilutions for use

1 : 50 000 with alkaline phosphatase (WB)

Molecular weight (expected | аpparent)

70 kDa

Verified reactivity

Cucurbita pepo, Pisum sativum, Triticum sp.

Possible reactivity

di and monocots

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

This antibody can inhibit protein import into organelles in vitro.

References

Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242

Scientific context

The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins.

Notes

This antibody will not react with bip or CSS1. Tested on 2D gels antibody reactos strongly with two different proteins, hsc70 and hsp70.

Protein number

Refer to NCBI

TAIR number

Refer to NCBI