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HSP70, salmonid heat shock protein 70, Affinity purified

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Lyophilized in PBS pH 7.4

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Affinity purified serum

How to reconstitute

For reconstitution add 200 µl of sterile water

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB), immunoprecipiation (IP), immunohistochemistry (IHC)

Connected products

collection of antibodies to fish related targetsSecondary antibodies

Recommended dilutions for use

1:15 000 with standard ECL (WB), 5 µg of antibodies in reaction mixture (IP), 1: 100 (IHC)

Molecular weight (expected | аpparent)

70 kDa

Verified reactivity

rainbow trout (Oncorhynchus mykiss)

Possible reactivity

atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis)

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive.

References

Curie et al. (2008). β-Adrenergic Stimulation Enhances the Heat-Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.

Scientific context

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Notes

immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

Protein number

B5X4Z3

TAIR number

Refer to NCBI

Test

A high affinity purification column was use to purify HSP70, salmonid heat shock protein 70, by agisera by chromatographic size exclusion.

Description

1 of protein A or G purified by agisera will give more specificity than crude serum.