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Rat HSP47 (Heat Shock Protein 47) ELISA Kit

Estimated Turnaround Time

6-11 business days

Target Name

NA

Target's alterntive name

HSP47

Species Reactivity

Rat

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

1.56-100 ng/mL

Sensitivity

0.94 ng/mL

Precision

NA

Detection Method

Colorimetric

Assay Time

4.5 hours

Application

For research use only. Not for diagnostic procedures.

Assay Type

Sandwich

Shipping Conditions

Ice packs

Storage Temperature

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Rat HSP47 (Heat Shock Protein 47) ELISA Kit before 6 months

Specificity

NA

Cross Activity

No significant cross-reactivity or interference was observed.

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP47 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP47 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP47, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSP47. The concentration of Rat HSP47 in samples can be calculated by comparing the OD of the samples to the standard curve.

Stability

NA

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Research Area

Cancer, Signal transduction

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus