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Human HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit

Estimated Turnaround Time

6-11 business days

Target Name

NA

Target's alterntive name

HSP-70/HSPA9

Species Reactivity

Human

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

0.78-50 ng/mL

Sensitivity

0.47 ng/mL

Precision

NA

Detection Method

Colorimetric

Assay Time

4.5 hours

Application

For research use only. Not for diagnostic procedures.

Assay Type

Sandwich

Shipping Conditions

Ice packs

Storage Temperature

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Human HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit before 6 months

Specificity

NA

Cross Activity

No significant cross-reactivity or interference was observed.

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-70 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-70, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-70. The concentration of Human HSP-70 in samples can be calculated by comparing the OD of the samples to the standard curve.

Stability

NA

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Research Area

Cancer, Metabolism, Neuroscience, Signal transduction

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED