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Multi-species Heat Shock Protein 70 (HSP70) ELISA Kit

Estimated Turnaround Time

6-11 Business days

Target Name

Heat Shock Protein 70

Target's alterntive name

DnaK, 70 Kilodalton Heat Shock Proteins

Species Reactivity

Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep)

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Detection Range

3.12-200 ng/mL

Sensitivity

1.27 ng/mL

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 70 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 70 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Detection Method

Colorimetric

Assay Time

3 hours

Application

For research use only. Not for diagnostic procedures.

Assay Type

Double-antibody Sandwich

Shipping Conditions

Ice packs

Storage Temperature

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Multi-species Heat Shock Protein 70 (HSP70) ELISA Kit before 8 months

Specificity

This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70

Cross Activity

No significant cross-reactivity or interference between Heat Shock Protein 70 and analogues was observed.

Test Principle

No significant cross-reactivity or interference between Heat Shock Protein 70 and analogues was observed.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Research Area

Tumor immunity, Infection immunity, Developmental science

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.