Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in different cell types and tissues (this protein has also been referred to as the Estrogen-Regulated 24 kDa protein, hsp25 and hsp28). Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and post-translational level. In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. Human HSP27 shares greater than 50% homology with the low molecular weight Drosophila HSP and mammalian crystalline lens protein. Because of the estrogen responsive nature of HSP27, the protein has been extensively studied in human estrogen responsive tissue such as cervix, endometrium and breast tissue. Recently, Hsp27 has been implicated as a regulator of breast tumor blood vessel growth, as an anti-inflammatory agent, and important for sensory neuron survival following axotomy.
HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067
Protein A Column
Prokaryotic the full length human hsp27 protein.
ELISA, Western Blot, immuno-histo-chemistry, immuno-cyto-chemistry, immuno precipitation
HSPB1 antibody can be used in immunohistochemistry starting at 35 ug/mL.
0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store HSPB1 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
heat shock 27kDa protein 1
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.