Grp78, is a 78 kDa glucose-regulated protein, also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins. Grp78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. Grp78 protein levels have been shown to be a reliable biomarker of hypoglycermia as well as serving a neuroprotective function in neurons exposed to glutamate and oxidative stress. Grp78 levels are reduced in the brains of Alzheimer's Disease patients and decreased expression of Grp78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. The induction of the Grp78 protein has been associated with the development of drug-resistance to antitumor drugs.
HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78
Protein G Column
HSPA5 antibody was made against amino acids 550 - 600 of HSPA5 (Human).
Western Blot, immuno-histo-chemistry
HSPA5 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 ug/mL, and immunofluorescence starting at 10 ug/mL.
PBS, 0.2% gelatin, 0.05% sodium azide.
HSPA5 antibody can be stored short term 4 grades C. For long term storage aliquot and store at -20 grades C. As with all antibodies avoid freeze/thaw cycles.
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.