HSPB1, like the other heat shock proteins, is part of a complex system of molecular chaperones in epidermal keratinocytes.
HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102
Antibody is purified by protein A chromatography method.
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSPB1.
ELISA, Western Blot, immuno-histo-chemistry
HSPB1 antibody can be used for detection of HSPB1 by ELISA at 1:62500. HSPB1 antibody can be used for detection of HSPB1 by western blot at 1.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store HSPB1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
heat shock 27kDa protein 1
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.