HSPE1 is a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner.
HSPE1, CPN10, GROES, HSP10, EPF
Antibody is purified by peptide affinity chromatography method.
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSPE1.
ELISA, Western Blot
HSPE1 antibody can be used for detection of HSPE1 by ELISA at 1:62500. HSPE1 antibody can be used for detection of HSPE1 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store HSPE1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
heat shock 10kDa protein 1
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.