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HSP26.5, Heat shock protein 26.5 (mitochondrial)

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Lyophilized

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HSP26.5, mitochondrial, UniProt: Q9SSQ8, TAIR: At1g52560

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Affinity purified serum

How to reconstitute

For reconstitution add 25 µl of sterile water.

Storage condition

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

Western Blot (WB)

Connected products

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 chicken antibodyAS08 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibodyAS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7AS11 1628 | anti-HSP18.5 | class IV heat shock protein, rabbit antibodyAS08 285 | anti-HSP21 | chloroplastic heat shock protein, rabbit antibodyAS15 2980 | anti-HSP23.6 | heat shock protein 23.6 (mitochondrial), rabbit antibodycollection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

26.5 | 23 kDa

Verified reactivity

Arabidopsis thaliana

Possible reactivity

Glycine soja

No reactivity

to be determined

Supplementary information

Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

References

To be added when available, antibody released in May 2016.

Scientific context

HSP26.5 | heat shock protein 26.5 (mitochondrial) belongs to small heat shock protein family and is localized in mitochondria.

Notes

Detection on total extracts needs to be optimized.Antibody is recognizing HSP23.6 synthesized in vitro using the PURExpress in Vitro Protein Synthesis Kt (NEB).

Protein number

Q9SSQ8

TAIR number

AT1G52560

Tissue

mitochondrial