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HSP23.5, Heat shock protein 23.5 (mitochondrial)

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Lyophilized

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HSP23.5, UniProt: Q9FGM9, TAIR: AT5G51440

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Affinity purified serum in PBS pH 7.4.

How to reconstitute

For reconstitution add 25 µl of sterile water.

Storage condition

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibodyAS08 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibodyAS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7collection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 500 (WB)

Molecular weight (expected | аpparent)

23.5 kDa

Verified reactivity

Arabidopsis thaliana recombinant HSP23.5

Possible reactivity

Arabidopsis thaliana

No reactivity

Chlamydomonas reinhardtii

Supplementary information

Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

References

To be added when available, antibody released in September 2016.

Scientific context

HSP23.5 | heat shock protein 23.5 (mitochondrial) belongs to a small heat shock protein family HSP20.

Notes

This antibody is recognizing recombinant HSP23.5 and reactivity on endogenous protein needs to be confirmed. High sensitivity reagents and enrichment are necessary due to low amounts of HSP23.5 in endogenous tissue.

Protein number

Q9FGM9

TAIR number

AT5G51440

Tissue

mitochondrial