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HSP18.5, class IV heat shock protein

Available ordering format

Lyophilized

Immunogen

recombinant Arabidopsis thaliana Hsp18.5

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 200 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS08 286 | anti-HSP16.6 | cytosolic class I heat shock protein 16.6, rabbit antibody for cyanobacterial HSP16.6AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6, rabbit antibodyAS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6, hen antibodyAS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7, rabbit antibodyAS08 285 | anti-HSP21 | chloroplastic heat shock protein, rabbit antibodyPlant protein extraction buffer Secondary antibodies

Recommended dilutions for use

1 : 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

18.5 kDa

Verified reactivity

Arabidopsis thaliana

Possible reactivity

dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays

No reactivity

no confirmed exceptions from predicted reactivity are currently known

Supplementary information

hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

References

to be added when available.

Scientific context

HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein

Notes

As hsp18.5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature. Normally I heat stress the seedling on a sealed agar plate for 2 hours. This assures that the humidity around the plant is very high. Low humidity can allow the plant to cool down through transpiration. If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment. Heat stressing plants in microphage tubes does not work that well.

Protein number

Refer to NCBI

TAIR number

AT2G19310