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HSP70/HSC70, heat shock protein 70

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Lyophilized

Immunogen

KLH-conjugated C-terminal synthetic peptide conserved in hsc/hsp70 sequences from a wide range of animal species

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 200 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

collection of antibodies to fish related targetsSecondary antibodies

Recommended dilutions for use

1:10 000 with ECL Advance (WB)

Molecular weight (expected | аpparent)

70 kDa

Verified reactivity

Arabidopsis thaliana, Acanthamoeba castellanii (amoeba), Dictyostelium discoideum, frog-heart, Frog-skeletal muscle, Frog-liver, Caenorhabditis elegans, salmon (Salmo salar) , rainbow trout (Oncorhynchus mykiss), cow, chicken, pig, rat, seal, mummichog

Possible reactivity

atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis)

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

not available at the moment

References

Chandra et al. (2012). Sustained high temperature increases the vitellogenin response to 17 alpha-ethynylestradiol in mummichog (Fundulus heteroclitus). Aquatic toxicology.

Scientific context

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. A killifish is a large family of various oviparous (egg-laying) cyprinodontiform fish including 1270 different species.

Notes

Chosen peptide sequence is also conserved in several fish species including: DQ202278.1 HSC70 Fundulus, DQ202279.1 HSP70-1 Fundulus, DQ202280.1 HSP70-2 Fundulus, BT059361.1 HSC70 Salmo salar (atlantic salmon), AB092839.2 HSP70 Carassius auratus (goldfish), BC056709.1 HSP70 Danio rerio (zebrafish) and other animal HSP70 proteins

Protein number

Refer to NCBI

TAIR number

Refer to NCBI