HSP90-1, heat shock protein 90-1

HSP90-1, heat shock protein 90-1

Size

50 µl

Product ID

AS08 346

Cost

434 EUR

Available ordering format

Lyophilized

Immunogen

Full length recombinant Hsp90-1 from Arabidopsis thaliana, UniProt: P27323, TAIR:AT5G52640.

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 50 µl of sterile water

Storage condition

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

Western blot (WB)

Connected products

Collection of antibodies to plant HSP proteinsPlant and algal protein extraction bufferSecondary antibodies

Recommended dilutions for use

1: 3000 with standard ECL (WB)

Molecular weight (expected | аpparent)

80.6 | 95 kDa (Arabidopsis thaliana)

Verified reactivity

Arabidopsis thaliana, Solanum lycopersicum, Zea mays

Possible reactivity

Glycine max, Hordeum vulgare, Micromonas pulsilla, Nicotiana benthamina, Nicotiana tabacum, Oryza sativa, Ostreococcus lucimarinus, Physcomitrella patens, Populus balsamifera, Ricinus communis, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera

No reactivity

No confirmed exceptions from predicted reactivity known in the moment.

Supplementary information

To be added when available.

References

Ghandi et al. (2016). Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperature. Sci Rep. 2016 Jan 21;6:19715. doi: 10.1038/srep19715 Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314. Moshe et al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275. Svozil et al. (2015). Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues. Front Plant Sci. 2015 May 28;6:376. doi: 10.3389/fpls.2015.00376. eCollection 2015. Tillmann et al. (2014). Hsp90 is involved in the regulation of cytosolic precursor protein abundance in tomato. Mol Plant. 2014 Oct 20. pii: ssu113. Svozil et al. (2014). Protein abundance changes and ubiquitylation targets identified after inhibition of the proteasome with Syringolin A. Mol Cell Proteomics. 2014 Apr 13. Finka et al. (2012). Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. Plant Cell, June 2012.

Scientific context

HSP90-1 (heast shock protein 90-1) is an isoform involved in response to bacterium, arsenic and heat. Synonymes: ATHS83; ATHSP90.1; F6N7.13; F6N7_13; HEAT SHOCK PROTEIN 81-1; HEAT SHOCK PROTEIN 83; HEAT SHOCK PROTEIN 90.1; HSP81-1; HSP81.1; HSP83.

Notes

This product can be sold containing sodium azide if requested.Antibody is recognizing both, heat inducible Hsp90-1 and constitutive isofrom Hsp90-2. Both proteins have ca. 85 % similarity.

Protein number

P27323

TAIR number

AT5G52640

Heat-shock Proteins!