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HSP101, ClpB heat shock protein, C-terminal

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Lyophilized

Immunogen

Recombinant protein of Arabidopsis thaliana Hsp101/ClpB, C-terminal 145 amino acids P42730, At1g74310

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 200 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS08 344 | anti-ClpB1 ATP-dependent chaperone, rabbits antibodiesAS08 355 |anti-ClpB2 ATP-dependent chaperone, rabbit antibodiesAS9 459 | anti-ClpB-P | ClpB3, rabbit antibodiesAS01 001 | anti-ClpC | chloroplastic form of HSP100, rabbit antibodiesAS13 2655 | anti-ClpP6 | chloroplast ClpP6 proteolytic subunit, rabbit antibodiesAS15 3077 | anti-pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1, rabbit antibodiesAS07 253 | anti-HSP101 | ClpB heat shock protein, N-terminal, rabbit antibodiesAS08 355 | anti-slr0156 | ATP-dependent chaperone clpB, rabbit antibodiesAS08 344 | anti-slr1641 | ATP-dependent chaperone clpB, rabbit antibodiesAS08 290 | anti-HSP101 | nuclear/cytoplasmatic heat shock protein, rabbit antibody with reactivity to Zea mayscollection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

101 kDa

Verified reactivity

Arabidopsis thaliana, Agave tequilana, Cucumis sativus L. var Krak

Possible reactivity

Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Triticum aestivum, Vitis vinifera

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

to be added when available

References

Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.

Scientific context

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.

Notes

None

Protein number

P42730

TAIR number

AT1G74310