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HSP16.6, Class I heat shock protein 16.6 (cytosolic)

Available ordering format

Lyophilized

Immunogen

Recombinant protein. Synechocystis PCC 6803 Hsp16.6 CI (class one) P72977

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 100 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS08 350 | anti-DnaK2 | heat shock protein 70-2 (HSP70-2)collection of antibodies to heat shock proteins

Recommended dilutions for use

1: 5000 with standard ECL (WB)

Molecular weight (expected | аpparent)

16.6 kDa

Verified reactivity

Synechocystis sp. PCC 6803

Possible reactivity

Synechococcus sp., Thermosynechococcus sp.

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

on a gradient gel Hsp16.6 runs closer to 11 kDa

References

Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.

Scientific context

Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

Notes

This product can be sold containing proclin if requested

Protein number

P72977

TAIR number

Refer to NCBI