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HSP21, Chloroplastic heat shock protein

Available ordering format

Lyophilized

Immunogen

Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana hsp21 P31170, At4g27670

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 50 µl of sterile water.

Storage condition

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

Western Blot (WB)

Connected products

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibodyAS08 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibodyAS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7collection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 3000 with standard ECL (WB)

Molecular weight (expected | аpparent)

25 | 21 kDa

Verified reactivity

Arabidopsis thaliana, Nicotiana tabacum

Possible reactivity

Arabidopsis thaliana

No reactivity

Chlamydomonas reinhardtii

Supplementary information

Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

References

Lämke et al. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J. 2016 Jan 18;35(2):162-75. doi: 10.15252/embj.201592593. Epub 2015 Dec 9.Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.Hai-Dong Yu et al. (2012). Downregulation of Chloroplast RPS1 Negatively Modulates Nuclear Heat-Responsive Expression of HsfA2 and Its Target Genes in Arabidopsis. Plos Genetics.

Scientific context

HSP21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

Notes

Recommended load per well 20 µg of total proteinThis product can be sold containing proClin if requested.

Protein number

P31170

TAIR number

AT4G27670