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HSP17.6, cytosolic class I heat shock protein 17.6

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Liquid in PBS pH 8.0, 0.02% sodium azide

Immunogen

Recombinant protein derived from a sequence Arabidopsis thaliana HSP17.6 Ci (class one) P13853, At1g53540

Raised in

Hen

Clonality

Polyclonal

Clone

Polyclonal

Purification

Total IgY

How to reconstitute

See included datasheet or contact our support service

Storage condition

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7collection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

17.6 kDa

Verified reactivity

Arabidopsis thaliana

Possible reactivity

n.a.

No reactivity

no confirmed exceptions from predicted reactivity known at the moment

Supplementary information

There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.

References

Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.

Scientific context

Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Notes

total IgY concentration is 32.2 µg/µl

Protein number

P13853

TAIR number

AT1G53540