Click to buy
HSP17.7, cytosolic class II heat shock protein 17.7

Available ordering format

Lyophilized

Immunogen

full length recombinant protein produced in E. coli and purified by conventional methods (no affinity tag). Arabidopsis thaliana Hsp17.7 CII (class two) O65376, At1g12030

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 200 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibodyAS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibodycollection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

17.7 kDa

Verified reactivity

Arabidopsis thaliana, Agave tequiliana var. Weber, Cucumis sativus, Medicago sativa, Pinellia ternata, Silene vulgaris

Possible reactivity

higher plants

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

to be added when available

References

Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1-12. Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244. Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.

Scientific context

Hsp17.7 belongs to a family of class II of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Notes

None

Protein number

O65376

TAIR number

AT1G12030