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HSP17.6, Cytosolic class I heat shock protein 17.6

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Lyophilized

Immunogen

Recombinant protein. Arabidopsis thaliana Hsp17.6 CI (class one) P13853, At1g53540

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 50 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibodyAS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7collection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

17.6 kDa

Verified reactivity

Arabidopsis thaliana, Agave tequilana var. Weber, Cucumis sativus, Iris pumilla (perennial monocot), Pinellia ternata, Pinus sylvestris, Silene vulgaris

Possible reactivity

higher plants

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.

References

Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print] Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression. Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)Florentin et al. (2013). Stress induces plant somatic cells to acquire some features of stem cells accompanied by selective chromatin reorganization. Dev. Dyn. Oct; 242(10):1121-33.

Scientific context

Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Notes

None

Protein number

P13853

TAIR number

AT1G53540