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HSP101, ClpB heat shock protein, N-terminal

Available ordering format

Lyophilized

Immunogen

Recombinant Hsp101 N-terminal derived from the sequence of Arabidopsis thaliana Hsp101 protein P42730, At1g74310

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 50 µl of sterile water.

Storage condition

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS08 344 | anti-ClpB1 ATP-dependent chaperone, rabbits antibodiesAS08 355 |anti-ClpB2 ATP-dependent chaperone, rabbit antibodiesAS9 459 | anti-ClpB-P | ClpB3, rabbit antibodiesAS01 001 | anti-ClpC | chloroplastic form of HSP100, rabbit antibodiesAS13 2655 | anti-ClpP6 | chloroplast ClpP6 proteolytic subunit, rabbit antibodiesAS15 3077 | anti-pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1, rabbit antibodiesAS08 287 | anti-HSP101 | ClpB heat shock protein, C-terminal, rabbit antibodiesAS08 355 | anti-slr0156 | ATP-dependent chaperone clpB, rabbit antibodiesAS08 344 | anti-slr1641 | ATP-dependent chaperone clpB, rabbit antibodiescollection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

101 kDa

Verified reactivity

Arabidopsis thaliana, Agave tequilana, Solanum lycopersicum, Thellungiella salsuginea

Possible reactivity

dicots including: Glycine max, Vitis vinifera, monocots including: Oryza sativa

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

To be added when available

References

Lämke et al. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J. 2016 Jan 18;35(2):162-75. doi: 10.15252/embj.201592593. Epub 2015 Dec 9.Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]Muench et al. (2016). Reactive electrophilic oxylipins trigger a heat stress-like response through HSFA1 transcription factors. J of Exp. Botany,10.1093/jxb/erw376.Fragkostefanakis et al. (2016). HsfA2 controls the activity of developmentally and stress-regulated heat stress protection mechanisms in tomato male reproductive tissues. Plant Physiol. 2016 Feb 25. pii: pp.01913.2015.Mishra et al. (2015). Characterization of 5'UTR of rice ClpB-C/Hsp100 gene: evidence of its involvement in post-transcriptional regulation. Cell Stress Chaperones. 2015 Nov 6.Kumar et al. (2015). Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress. Saudi Journal of Biol. Sciences, March 2015.Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression. Sci Rep. 2015 Jan 26;5:8030. doi: 10.1038/srep08030.Mishra and Grover (2014). Intergenic Sequence between Arabidopsis Caseinolytic Protease B-Cytoplasmic/Heat Shock Protein100 and Choline Kinase Genes Functions as a Heat-Inducible Bidirectional Promoter 1. Plant Physiol. 2014 Nov;166(3):1646-58. doi: 10.1104/pp.114.250787.Gamburg et al. (2014). The Relationship between the Differences in Frost Resistance of Arabidopsis and Thellungiella and Heat Shock Proteins and Dehydrins. Russian J. Plant Physiol. May 2014, Volume 61, Issue 3, pp 318-323 Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)

Scientific context

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. HSP101 is a cytosolic heat shock protein required for acclimation to high temperature.

Notes

None

Protein number

P42730

TAIR number

AT1G74310