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HSP, Heat shock cognate protein 70, Affinity purified

Available ordering format

Lyophilized in PBS pH 7.4

Immunogen

KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Affinity purified serum

How to reconstitute

For reconstitution add 50 µl of sterile water

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

Western blot (WB), immunoprecipitation (IP)

Connected products

Secondary antibodies

Recommended dilutions for use

1: 1000 with standard ECL (WB), 1: 10 000 with ECL Advance (GE Healthcare), 1: 1000 (IP)

Molecular weight (expected | аpparent)

70 kDa

Verified reactivity

fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Gloeophyllum protractum, G. sepiarium, G. carbonarium, Oligoporus sericiomollis, Phlebia cornea, Junghunia luteoalba

Possible reactivity

vertebrates including bovine, hen, mouse, rat; insects including Drosophila melanogaster

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

this antibody is not suitable for work with samples from higher plants

References

French et al. (2015). High survivorship after catch-and-release fishing suggests physiological resilience in the endothermic shortfin mako shark (Isurus oxyrinchus). Conservation Physiology, Vol 3, Issue 1, 10.1093/conphys/cov044

Scientific context

Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1, HSP70-1/HSP70-2, Heat shock 70 kDa protein 8

Notes

for detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371

Protein number

P08107 , P11142

TAIR number

Refer to NCBI

Test

A high affinity purification column was use to purify HSP, Heat shock cognate protein 70, by agisera by chromatographic size exclusion.

Description

1 of protein A or G purified by agisera will give more specificity than crude serum.