HSP70/HSC70, Heat shock protein 70/Heat shock cognate protein 70 (serum)

HSP70/HSC70, Heat shock protein 70/Heat shock cognate protein 70 (serum)

Size

100 µl

Product ID

AS05 083

Cost

533 EUR

Available ordering format

Lyophilized

Immunogen

KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 100 µl of sterile water

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

Western blot (WB), immunoprecipitation (IP)

Connected products

Secondary antibodies

Recommended dilutions for use

1: 1000 with standard ECL (WB), 1: 10 000 with ECL Advance (GE Healthcare), 1: 1000 (IP)

Molecular weight (expected | аpparent)

70 kDa

Verified reactivity

fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Catostomus commersonii, Gloeophyllum protractum, G. sepiarium, G. carbonarium, Oligoporus sericiomollis, Phlebia cornea, Junghunia luteoalba

Possible reactivity

vertebrates including bovine, hen, mouse, rat; insects including Drosophila melanogaster

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

This antibody is recognizing both, the inducible and the constitutive Hsp70.

References

MacLellan et al. (2015). Chaperone roles for TMAO and HSP70 during hyposmotic stress in the spiny dogfish shark (Squalus acanthias). J Comp Physiol B. 2015 Jun 7. Bessemer et al. (2014). Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology. 2014 Nov 27:1-10.Gorovits et al. (2013). Recruitment of the host plant heat shock protein 70 by tomato yellow leaf curl virus coat protein is required for virus infection. PLoS Once, July 23;8(7).

Scientific context

Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1, HSP70-1/HSP70-2, Heat shock 70 kDa protein 8

Notes

for detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371

Protein number

Refer to NCBI

TAIR number

Refer to NCBI

Tissue

serum

Description

Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

Group

Sera

About

Serum should be stored at +5°C

Heat-shock Proteins!