HSP90, Heat shock protein 90

HSP90, Heat shock protein 90

Size

100 µl

Product ID

AS05 063

Cost

534 EUR

Available ordering format

Lyophilized

Immunogen

KLH-conjugated synthetic peptide chosen from a highly conserved region of hsp90 found in both the alpha P07900 and beta P08238 form of the protein. The target peptide is perfectly conserved in animals.

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 100 µl of sterile water

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

Western blot (WB)

Connected products

AS05 061 | anti-salmonid HSP70AS05 061A | anti-salmonid HSP70, suitable for immunoprecipitationAS05 062 | anti-salmonid HSC70AS05 083A | anti-HSP70/HSC70 global antibody, affinity purifiedSecondary antibodies

Recommended dilutions for use

1: 5 000 with standard ECL (WB)

Molecular weight (expected | аpparent)

84-86 kDa

Verified reactivity

salmon, human

Possible reactivity

fishes, hen, mammals

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

in salmonid fish a cross-reactive band at approximately 40 kDa is observed; antibody will also detect a human recombinant HSP90 proteinAntibody is reacting strongly with recombinant HSP90 from HELA cells.

References

Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015. Liu et al. (2014). Spermidine Enhances Waterlogging Tolerance via Regulation of Antioxidant Defence, Heat Shock Protein Expression and Plasma Membrane H+-ATPase Activity in Zea mays. J. Agronomy and Crop Science, Article first published online: 1 APR 2014, DOI: 10.1111/jac.12058. Chandra et al. (2012). Sustained high temperature increases the vitellogenin response to 17 alpha-ethynylestradiol in mummichog (Fundulus heteroclitus). Aquatic toxicology.

Scientific context

HSP90 is a ubiquitous chaperone involved in numerous cellular processes. Members of this family of proteins play important roles in allowing a selected group of intracellular signaling molecules reach and maintain functionally active conformations.Alternative names: for hsp90 alpha: HSP 86, renal carcinoma antigen NY-REN-38, for hsp90 beta: hsp84

Notes

None

Protein number

P07900 , P08238

TAIR number

Refer to NCBI

Heat-shock Proteins!