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HSC70, salmon heat shock cognate protein 70

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Lyophilized

Immunogen

KLH-conjugated synthetic peptide specific to the hsc70 of salmonid fish B5X3U6_SALSA

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 100 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

AS05 061 | anti-salmonid HSP70, rabbit antibodyAS05 061A | anti-salmonid HSP70, rabbit antibody suitable for immunoprecipitationAS05 063 | anti-salmonid HSP90, rabbit anitbodySecondary antibodies

Recommended dilutions for use

1:5 000 with ECL Advance (GE Healthcare) (WB)

Molecular weight (expected | аpparent)

72 | 70 kDa

Verified reactivity

salmon

Possible reactivity

bovine, human, mouse, rat , Zebra fish

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

not available at the moment

References

LeBlanc et al. (2011). Chronic social stress impairs thermal tolerance in the rainbow trout (Oncorhynchus mykiss). J Exp Biol. 15;214:1721-31.Rendell et al. (2006). Development-dependent differences in intracellular localization of stress proteins (hsps) in rainbow trout, Oncorhynchus mykiss, following heat shoc. Comp. Biochem and Physiol 1:238-252.

Scientific context

Heat Shock Cognate Protein 70 (Hsc70) is crucial for the chaperoning function of unstressed cells.

Notes

None

Protein number

B5X3U6

TAIR number

Refer to NCBI