100 µl
AS05 061
550 EUR
Lyophilized
KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.
Rabbit
Polyclonal
Polyclonal
Serum
For reconstitution add 100 µl of sterile water.
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
western blot (WB)
collection of antibodies to fish related targetsSecondary antibodies
1:5 000 with standard ECL (WB)
70 kDa
rainbow trout (Oncorhynchus mykiss), brook trout gills (Salvelinus fontinalis)
atlantic salmon (Salmo salar),
no confirmed exceptions from predicted reactivity known in the moment
not available at the moment
Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015.Templeman et al. (2014). Linking physiological and cellular responses to thermal stress: β-adrenergic blockade reduces the heat shock response in fish. J Comp Physiol B, April 2014.
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.
The antibody is very specific for salmonid inducible form - hsp70. It does not cross-react with hsc70. It does not detect hsp70 from other species.
B5X4Z3
Refer to NCBI
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