HSP70, salmonid heat shock protein 70

HSP70, salmonid heat shock protein 70

Size

100 µl

Product ID

AS05 061

Cost

550 EUR

Available ordering format

Lyophilized

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 100 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB)

Connected products

collection of antibodies to fish related targetsSecondary antibodies

Recommended dilutions for use

1:5 000 with standard ECL (WB)

Molecular weight (expected | аpparent)

70 kDa

Verified reactivity

rainbow trout (Oncorhynchus mykiss), brook trout gills (Salvelinus fontinalis)

Possible reactivity

atlantic salmon (Salmo salar),

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

not available at the moment

References

Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015.Templeman et al. (2014). Linking physiological and cellular responses to thermal stress: β-adrenergic blockade reduces the heat shock response in fish. J Comp Physiol B, April 2014.

Scientific context

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Notes

The antibody is very specific for salmonid inducible form - hsp70. It does not cross-react with hsc70. It does not detect hsp70 from other species.

Protein number

B5X4Z3

TAIR number

Refer to NCBI

Heat-shock Proteins!