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Rat HSP-27 (Heat Shock Protein 27) CLIA Kit

Detection Type

Colormetric

Target Species

Rat

Target Synonym

NA

Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-27 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-27 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSP-27 . You can calculate the concentration of Rat HSP-27 in the samples by comparing the RLU value of the samples to the standard curve.

Sensitivity

0.09ng/mL

Detection Range

0.16~10ng/mL

Target Name

HSP-27

UNIProt ID

NA

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Rat HSP-27 (Heat Shock Protein 27) CLIA Kit

Type

Sandwich

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus