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Porcine HSP-70/HSPA9(Heat Shock Protein 70) EIA Kit

Detection Type

Colormetric

Target Species

Porcine

Target Synonym

HSPA9, CSA, GRP-75, GRP75, HSPA9B, MOT, MOT2, MTHSP75, PBP74

Test principle

This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Porcine HSP-70/HSPA9. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine HSP-70/HSPA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine HSP-70/HSPA9. You can calculate the concentration of Porcine HSP-70/HSPA9 in the samples by comparing the OD of the samples to the standard curve.

Sensitivity

0.38ng/mL

Detection Range

0.63~40ng/mL

Target Name

HSP-70/HSPA9

UNIProt ID

F1RGJ3

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Porcine HSP-70/HSPA9(Heat Shock Protein 70) EIA Kit

Type

Sandwich

Description

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.

Properties

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay