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Recombinant Human Heat Shock Protein β-11/HSPB11 Protein(N-6His)

Synonym

Heat Shock Protein Beta-11; Hspb11; Placental Protein 25; PP25; HSPB11; C1orf41

Activity

NA

Sequence

Met1-Ser144

Fusion tag

N-6His

Accession

Q9Y547

Expressed Host

E.coli

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Purity

＞95% as determined by reducing SDS-PAGE.

Endotoxin

＜1.0 EU per µg as determined by LAL test.

Stability and Storage

Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles

Mol Mass

18.5 kDa

AP Mol Mass

21 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 2mM DTT, 10% Glycerol, pH 8.0.

Background

Heat Shock Protein β-11 (HSPB11) is a stress-responsive protein that is required to deal with proteotoxic stresses. HSPB11 is composed of an IFT complex B composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20 and is detected in placenta. HSPB11 has beeb shown to form oligomeric complexes and prevent the aggregation of in vitro denaturated aldolase and glyceraldehyde-3-phosphate dehydrogenase in accordance with the chaperone model of HSPB1 and HSPB5. HSPB11 overexpression protected against etoposide-induced cell death that correlated with a decreased release of mitochondrial Cytochrome C into the cytosol. Inhibition of HSP90 function completely abrogated the protective effect of HSPB11. This data suggests that at least in the case of HSPB11, interaction with other chaperone machines besides HSPA1A may contribute to functional specificity and cellular functioning.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Source

Recombinants or rec. proteins

Group

recombinants